Norlab

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Nucleic acids

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Overview

Characterization of nucleic acids by Field-Flow Fractionation (FFF)
During the past decades, FFF has been applied successfully for the separation, characterization and fractionation of a wide variety of large-size materials, including nucleic acids.

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Postnova AF2000 MT mid temperature MultiFlow FFF system
The system can be configured for Asymmetric Flow Field-Flow Factionation (AF4), Hollow Fiber Flow Field-Flow Factionation (HF5) and Size Exclusion Chromatography (SEC).

Postnova CF2000 Centrifugal FFF system
The system can separate and distinguish relative size differences of 5% only and can be used to monitor absolute size differences down to 1 nm. These unique features make it the ideal choice for any nanoparticle research and QC tasks.

Postnova 21-angle MALS detector - the ideal detector for ultra-high molar mass
The Postnova 21-angle MALS detector has been especially developed for coupling with FFF systems – to the opposite of previously available MALS detectors which were designed with chromatography (SEC/GPC) in mind, that is for smaller molar mass.

High sensitivity online Dynamic Light Scattering detector, special customized for use connected to FFF
Online DLS measurements using a special flow cell can be perfomed to obtain particle size (Rh) and molecular weight (Mw) distributions of particles and molecules separated by Postnova Asymmetric Flow FFF (AF4), Centrifugal FFF (CF3) and Thermal FFF (TF3) systems.

Application notes

Comparison of Omni soil DNA kit over Company M’s soil DNA isolation kit for DNA extraction from soil samples
The main objective of this study was to compare the performance of Omni's soil DNA kit (26-013G/B) to that of Company M’s soil DNA isolation kit in terms of DNA yield and quality, as well as amplification potential and sensitivity of detection using real-time PCR.

Evaluation of humic acid removal from DNA purified via Omni soil DNA purification kit
Date of creation: 
24 January 2020
Library code: 
12805
Evaluation of sample disruption techniques for high throughput extraction of live escherichia coli and recombinant DNA from spinach
Date of creation: 
24 January 2020
Library code: 
12806
Genomic DNA extraction from plant tissues on the Omni Bead Ruptor 24
Date of creation: 
25 January 2020
Library code: 
12808
High-throughput genomic DNA and protein extraction from multiple tissue types using the Omni Bead Ruptor 96 bead mill homogenizer
Date of creation: 
25 January 2020
Library code: 
12810
Increased mRNA yield from skin samples using the Omni Bead Ruptor Elite
Date of creation: 
25 January 2020
Library code: 
12811
Mosquito DNA extraction and high throughput gene targeting on Omni Bead Ruptor 96 bead mill homogenizer
Date of creation: 
25 January 2020
Library code: 
12812
Purification and evaluation of total RNA from porcine skin on the Bead Ruptor Elite bead mill homogenizer
Date of creation: 
25 January 2020
Library code: 
12816
RNA extraction from Saccharomyces cerevisiae (yeast) on the Omni Bead Ruptor 24
Date of creation: 
25 January 2020
Library code: 
12819
Total genomic DNA and protein extraction from Escherichia coli K12 cells on the Omni Bead Ruptor 4
Date of creation: 
25 January 2020
Library code: 
12821
Literature

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

Date of creation: 
24 July 2017
Library code: 
10163
Date of creation: 
29 September 2015
Library code: 
10159
Date of creation: 
1 October 2015
Library code: 
10164
Multi-Angle Light Scattering detector (MALS) model 3621
Date of creation: 
27 July 2017
Library code: 
10220
Dynamic Light Scattering detector model Zetasizer Nano ZS173
Date of creation: 
1 October 2015
Library code: 
10221