Supported Liquid Extraction

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Overview

Sample preparation using Supported Liquid Extraction

The Supported Liquid Extraction (SLE) process is analogous to traditional liquid-liquid extraction (LLE) and utilizes the same water immiscible solvent systems for analyte extraction. However, instead of shaking the two immiscible phases together, the aqueous phase is immobilized on an inert diatomaceous earth based support material and the water immiscible organic phase flows through the support, alleviating many of the liquid handling issues associated with traditional LLE such as emulsion formation. As a result recoveries are often higher and demonstrate better reproducibility from sample to sample.

Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide
This guide describes how to develop supported liquid extraction methods using ISOLUTE SLE+ products, along with hints and tips for optimizing performance and extending the range of analytes that can be extracted.

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Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns
ISOLUTE SLE+ Supported Liquid Extraction plates and columns offer an efficient alternative to traditional Liquid Liquid Extraction (LLE) for bioanalytical sample preparation, extracting up to 10 mL of aqueous sample. ISOLUTE SLE+ provides high analyte recoveries, eliminates emulsion formation and reduces sample preparation time by half.

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Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology
This poster compares the performance of manual processing to a novel automated sample preparation system prior to GC/MS or LC-MS/MS analysis.

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Converting a liquid-liquid extraction method for vitamin D to a Supported Liquid Extraction plate format
The objective of this study was to determine feasibility of transferring a validated Liquid-Liquid Extraction (LLE) method to a Supported Liquid Extraction (SLE) method using Biotage ISOLUTE SLE+ 96-well plates (400 uL sample capacity). The laboratory supporting this study processes ~550 samples per month supporting vitamin D analysis in plasma and serum (mixed samples / not separated). During this evaluation, it was determined that sample processing time was reduced by ~50% for a de-identified patient set (n=30, split and processed by both methods). Towards high throughput analysis platforms, ISOLUTE SLE+ was demonstrated as a viable solution in the sample preparation workflow for vitamin D applications.

47 drugs of abuse that can be extracted with Supported Liquid Extraction (SLE)
This application note describes the extraction of 47 drugs of abuse from oral fluid matrix after sampling via Quantisal collection devices prior to UPLC-MS/MS analysis.

Biotage ISOLUTE SLE+ applications
The ISOLUTE SLE+ applications e-book is designed to give an easy to use overview of the wide range of ISOLUTE SLE+ applications available.

Biotage analytical sample preparation 2017
This catalog presents the extensive range of products for analytical sample preparation from Biotage.

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Application notes

Biotage ISOLUTE SLE+ applications
The ISOLUTE SLE+ applications e-book is designed to give an easy to use overview of the wide range of ISOLUTE SLE+ applications available.

Download Application note, Converting a Liquid-Liquid Extraction method for Vitamin D to a 96-well plate Supported Liquid Extraction format.pdf (373.95 KB)

Converting a liquid-Lliquid Extraction method for vitamin D to a Supported Liquid Extraction plate format

Date of creation:

30 December 2015

Library code:

10566

Download Application note, Extraction of 47 drugs of abuse from oral fluid by Biotage ISOLUTE SLE+ columns and plates.pdf (1.69 MB)

47 drugs of abuse that can be extracted with Supported Liquid Extraction (SLE)

Date of creation:

13 January 2016

Library code:

10582

Download Application note, Extraction of cocaine and metabolites from whole blood using ISOLUTE SLE+ prior to GC-MS analysis.pdf (687.88 KB)

Extraction of cocaine and metabolites from whole blood using ISOLUTE SLE+ prior to GC-MS analysis

Date of creation:

14 January 2016

Library code:

10583

Download Application note, Extraction of opiates from whole blood using SLE.pdf (698.56 KB)

Extraction of opiates from whole blood using ISOLUTE SLE+ prior to GC-MS analysis

Date of creation:

14 January 2016

Library code:

10585

Download Application note, Extraction of THC, Hydroxy-THC and Carboxy-THC from whole blood by SLE.pdf (686.18 KB)

Extraction of THC, Hydroxy-THC and Carboxy-THC from whole blood using ISOLUTE SLE+ prior to GC-MS analysis

Date of creation:

14 January 2016

Library code:

10587

Download Application note, Extraction of NBOMe designer drugs from oral fluid using ISOLUTE SLE+.pdf (1.39 MB)

Extraction of NBOMe designer drugs from oral fluid using ISOLUTE SLE+ prior to analysis by LDTD-MS/MS

Date of creation:

14 January 2016

Library code:

10588

Download Application note, Extraction of amphetamines and analogues from hair using ISOLUTE SLE+ prior to GC-MS analysis.pdf (875.53 KB)

Extraction of amphetamines and analogues from hair using ISOLUTE SLE+ prior to GC/MS analysis

Date of creation:

27 September 2017

Library code:

11288

Download Application note, Extraction of illicit drugs and pesticides from liver tissue using ISOLUTE SLE+ prior to GC-MS analysis.pdf (2.32 MB)

Extraction of illicit drugs and pesticides from liver tissue using ISOLUTE SLE+ prior to GC/MS analysis

Date of creation:

27 September 2017

Library code:

11289

Download Application note, Acidic, basic and neutral drug screen of hydrolyzed urine using supported liquid extraction prior to LC-MS-MS analysis.pdf (906.41 KB)

Acidic, basic and neutral drug screen of hydrolyzed urine using supported liquid extraction prior to LC-MS/MS analysis

Date of creation:

28 September 2017

Library code:

11291

Literature

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Download Technical note, Improve productivity and maximize analyte recovery with Biotage ISOLUTE SLE+ Supported Liquid Extraction plates and columns.pdf (344.44 KB)

Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns

Date of creation:

30 December 2015

Library code:

10563

Download Data sheet, Biotage ISOLUTE SLE+ 48-Well Supported Liquid Extraction plate.pdf (1.09 MB)

Biotage ISOLUTE SLE+ 48-well Supported Liquid Extraction plate

Date of creation:

30 December 2015

Library code:

10565

Download User guide, Biotage ISOLUTE SLE+ Supported Liquid Extraction.pdf (1.98 MB)

Biotage ISOLUTE SLE+ Supported Liquid Extraction user guide

Date of creation:

30 December 2015

Library code:

10568

Download Poster, Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology.pdf (937.72 KB)

Evaluation of novel automated sample preparation compared to manual processing in forensic toxicology

Date of creation:

22 August 2017

Library code:

11221

Download Technical note, Improve productivity and maximize analyte recovery with Biotage ISOLUTE SLE+ Supported Liquid Extraction plates and columns.pdf (344.44 KB)

Improve productivity and maximize analyte recovery with ISOLUTE SLE+ plates and columns

Date of creation:

30 December 2015

Library code:

10563

Download Data sheet, Biotage ISOLUTE SLE+ 48-Well Supported Liquid Extraction plate.pdf (1.09 MB)

Biotage ISOLUTE SLE+ 48-well Supported Liquid Extraction plate

Date of creation:

30 December 2015

Library code:

10565

Download Brochure, Biotage Extrahera Sample Preparation Automation System.pdf (2.67 MB)

Biotage Extrahera Sample Preparation Automation System

Date of creation:

3 December 2015

Library code:

10482

Download Brochure, Biotage Extrahera sample preparation automation system.pdf (4.48 MB)

Biotage Extrahera sample preparation automation system

Date of creation:

24 August 2017

Library code:

11231

Download Catalog, Biotage analytical sample preparation 2017.pdf (4.78 MB)

Biotage analytical sample preparation 2017

Date of creation:

22 August 2017

Library code:

11216

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