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Protein precipitation

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Overview

Protein precipitation by filtration with Biotage ISOLUTE PPT+ protein precipitation plates

  • ‘Solvent first’ methodology for maximizing precipitation efficiency
  • Eliminate cloudy extracts
  • No well blockage
  • No vortex mixing required
  • Optimized for automation
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Biotage ISOLUTE PPT+ protein precipitation plates
Sample preparation using ISOLUTE PPT+ Protein Precipitation Plates is fast and simple. The optimized filtration system in ISOLUTE PPT+ plates provides an easy to automate solution for efficient protein removal. The procedure is streamlined,with no awkward off-line steps.

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Sample preparation using ISOLUTE PPT+ protein precipitation plates
This chemistry data sheet describes the use of ISOLUTE® PPT+ Protein Precipitation Plates for the high
throughput sample preparation of biological fluids (plasma, serum or blood).

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Biotage Extrahera sample preparation automation system
Extrahera is a powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in plate or column formats.

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Biotage Extrahera Sample Preparation Automation System
The Extrahera is a powerful, user friendly automation system for Supported Liquid Extraction (SLE), Solid Phase Extraction (SPE), Phospholipid Depletion (PLD) and Protein Precipitation (PPT) based methods, in 96 and 48 well plate formats, or 1, 3 and 6 mL column formats.

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Biotage analytical sample preparation 2017
This catalog presents the extensive range of products for analytical sample preparation from Biotage.

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Application notes

Extraction of methylmalonic acid from serum using ISOLUTE PPT+ protein precipitation plates prior to LC-MS/MS analysis
This application note describes a simple ISOLUTE PPT+ protocol for the extraction of methylmalonic acid (MMA) from serum, demonstrating high, reproducible analyte recoveries with low protein content in the extracts.

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Literature

Biotage ISOLUTE PPT+ protein precipitation plates
Sample preparation using ISOLUTE PPT+ Protein Precipitation Plates is fast and simple. The optimized filtration system in ISOLUTE PPT+ plates provides an easy to automate solution for efficient protein removal. The procedure is streamlined,with no awkward off-line steps.

Readmore >>
PDF icon Download Data sheet, Sample preparation using ISOLUTE PPT+ protein precipitation plates.pdf (138.67 KB)
Sample preparation using ISOLUTE PPT+ protein precipitation plates
Date of creation: 
30 December 2015
Library code: 
10557
PDF icon Download Brochure, Biotage Extrahera Sample Preparation Automation System.pdf (2.67 MB)
Biotage Extrahera Sample Preparation Automation System
Date of creation: 
3 December 2015
Library code: 
10482
PDF icon Download Brochure, Biotage Extrahera sample preparation automation system.pdf (4.48 MB)
Biotage Extrahera sample preparation automation system
Date of creation: 
24 August 2017
Library code: 
11231
PDF icon Download User guide, Biotage Extrahera user guide - getting started.pdf (3.68 MB)
Biotage Extrahera user guide - getting started
Date of creation: 
25 August 2017
Library code: 
11235
PDF icon Download User guide, Biotage Extrahera user guide - installation and safety.pdf (1.17 MB)
Biotage Extrahera user guide - installation and safety
Date of creation: 
25 August 2017
Library code: 
11236
PDF icon Download User guide, Biotage Extrahera user guide - site requirements.pdf (468.01 KB)
Biotage Extrahera user guide - site requirements
Date of creation: 
25 August 2017
Library code: 
11237
PDF icon Download Catalog, Biotage analytical sample preparation 2017.pdf (4.78 MB)
Biotage analytical sample preparation 2017
Date of creation: 
22 August 2017
Library code: 
11216