Field-Flow Fractionation (FFF) and Size Exclusion Chromatography (SEC)

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Overview

Characterization of large molecules and particles by Field-Flow Fractionation (FFF)
FFF is a family of analytical techniques which are ideally suited for very gentle and high resolution separation and characterization of large molecules and particles, which traditional chromatographic techniques cannot perform.

Separation, characterization and fractionation of nearly any macromolecules or nanoparticles
The Postnova modular FFF platform can be configured for any single or combination of several of the five FFF subtechniques - Asymmetric Flow FFF (AF4), Hollow-Fibre Flow FFF (HF5), Electrical FFF (EAF4), Centrifugal FFF CF3) and Thermal FFF (TF3). Because of this flexibility, it can be used for the separation, characterization and fractionation of nearly any macromolecules or nanoparticles. The Postnova platform can be also configurated for Size Exclusion Chromatography (SEC or GPC). You can easily switch from FFF to SEC and vice versa. The NovaFFF software takes care of control, data acquisition and calculation.

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Postnova EAF2000 electrical asymmetrical flow FFF system
The Postnova EAF2000 system is based on the AF2000 asymmetrical flow FFF module, with an additional Electrical FFF module, a special EAF4 channel and a software module. The EAF2000 is ideal for many applications in the domains of biopharm, environment and nanomaterials.

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Postnova AF2000 MT mid temperature MultiFlow FFF system
The system can be configured for Asymmetric Flow Field-Flow Factionation (AF4), Hollow Fiber Flow Field-Flow Factionation (HF5) and Size Exclusion Chromatography (SEC).

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Postnova CF2000 Centrifugal FFF system
The system can separate and distinguish relative size differences of 5% only and can be used to monitor absolute size differences down to 1 nm. These unique features make it the ideal choice for any nanoparticle research and QC tasks.

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Postnova TF2000 Thermal FFF system
The system can separate simultaneously polymers and particles without the limitations associated with GPC/SEC. The separation takes place according to molecular weight AND chemical composition, thus making the TF2000 Series the ideal choice for difficult polymer separation.

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Postnova 21-angle MALS detector - the ideal detector for ultra-high molar mass
The Postnova 21-angle MALS detector has been especially developed for coupling with FFF systems – to the opposite of previously available MALS detectors which were designed with chromatography (SEC/GPC) in mind, that is for smaller molar mass.

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High sensitivity online Dynamic Light Scattering detector, special customized for use connected to FFF
Online DLS measurements using a special flow cell can be perfomed to obtain particle size (Rh) and molecular weight (Mw) distributions of particles and molecules separated by Postnova Asymmetric Flow FFF (AF4), Centrifugal FFF (CF3) and Thermal FFF (TF3) systems.

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Model 3310 viscometer detector
The Postnova model 3310 viscometer detector is a state-of-the-art intrinsic viscosity detector for Field-Flow Fractionation (FFF) and Size Exclusion Chromatography (GPC/SEC). It is based on the proven Wheatstone-Bridge design for measuring the intrinsic viscosity of macromolecules in solution. The instrument is designed in the modern and compact Postnova FFF detector design.

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Postnova model 8050 FFF fraction collector
Model 8050 employs the latest state-of-the-art robotic sample and fluid transfer technology such as a double needle, special wash routines for minimal carry over and direct fractionation into closed vials. It can be operated with different microwell plates, Eppendorf caps as well as chromatography and preparative vials. It is available as biocompatible and metal-free version.

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Application notes

Measurement of cancer biomarker enrichment in exosomes: separation using both AF4 and CF3
In this study the goal was to characterize the MCF-7 tumor exosome sample by size using asymmetrical flow field-flow fractionation (AF4) coupled to multi angle light scattering (MALS), and by mass using centrifugal field-flow fractionation (CF3).