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Field-flow fractionation with single particle ICP-MS as an online detector
This application note reports direct hyphenation of spICP-MS to the asymmetrical flow FFF (AF4) system for size- and number- based characterization of a mixture of gold nanoparticles.

Comparison of sensitivity: silica nanoparticles studied by Zetasizer Nano S vs. ZSP coupled online with centrifugal field-flow fractionation
In this application, SiO2 nanoparticles were used to show the separation efficiency of centrifugal FFF combined with online-DLS for silica nanoparticles. A 3:2:1 mixture of SiO2 NPs (w%) with average size diameters of 160 nm, 200 nm and 500 nm diluted in water (MilliQ, 10-18 MΩ) with 0.05% NovaChem100 (v/v) was prepared and injected into the FFF system.

Hyphenation of asymmetrical flow field-flow fractionation and ICP-MS for multi-element analysis and characterization of plasma proteins
In this study, asymmetrical flow FFF (AF4) was used to separate a mixture of human serum albumin - binding 63Cu and transferrin - binding 56Fe metal ions. The AF4 system separated the proteins by size and detected the metal ions by ICP-MS to obtain metal distributions of the protein mixture.

Centrifugal field-flow fractionation hyphenated with dynamic light scattering for size characterization of nanoparticle systems for biopharmaceutical applications
The hyphenation of centrifugal FFF with dynamic light scattering has proven to be an ideal tool for the investigation of particle sizes even in complex matrices such as e.g., cell medium. CF3-DLS thereby for the first time not only enabled the detection, but also the evaluation of the particle integrity as well as the monitoring of particle size alterations of PLGA NPs directly in cell medium over time.

Study of temperature stability, fragmentation and aggregation of recombinant antibodies with asymmetrical flow FFF and UV
In this study, Postnova investigated the influence of temperature on the stability, fragmentation and aggregation of recombinant antibodies. Thereby, they could clearly demonstrate the excellent applicability of AF4 coupled with UV as a monitoring tool for temperature-induced antibody decomposition revealing that the antibody itself is rather decomposed to fragments than to aggregates.

Analysis of fullerene clusters using asymmetrical flow FFF hyphenated with an Orbitrap high resolution with an APPI mass spectrometer
This note shows that it is possible to couple flow FFF online to an Orbitrap HRMS with an APPI chamber. This setup facilitates the identification of fullerene clusters in aqueous matrices directly after separation of the particles with asymmetrical flow FFF.

Capabilities of asymmetrical flow field-flow fractionation coupled with MALS for the detection of carbon nanotubes in soot and soil
Postnova evaluates a shape factor ρ, derived from AF4-MALS measurements, for its capabilities to detect MWCNTs in soot and soil.

Asymmetric flow FFF, analytical ultracentrifugation and size exclusion chromatography monoclonal antibody aggregate quantification
AF4 is a separation technique using an unpacked separation channel instead of a packed column. Lack of a stationary phase provides unique advantages, which are: no shear forces, matrix interaction-free sample separation, fast separation times and great flexibility with respect to the choice of solvent.

Postnova high temperature asymmetric flow field-flow fractionation AF2000 MultiFlow system
Because of several integrated patents, the Postnova AF2000 series platform is the most advanced AF4 technology available on the market today, making it the benchmark for the industry and delivering the maximum performance possible.

Postnova Shortix tubing cutter for fused silica capillary
The Shortix is an easy and fast to use tubing cutter for fused silica cappilary, saving money and time.

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