pdf

Extraction of opiates from human urine using ISOLUTE SLE+ 96 -well plates and columns
The methdon in this application note describes the use of ISOLUTE SLE+ supported liquid extraction plates (96 -well) and 1 mL sample volume columns to extract a range of opiates including opiate treatment analytes (methadone and its metabolite EDDP) from human urine.

Extraction of vitamin D metabolites from human serum using ISOLUTE SLE+ in 96-fixed well plate format prior to LC-MS/MS analysis
The method in this application note describes the use of ISOLUTE SLE+ plates for the efficient and simple extraction of the vitamin D metabolites 25-OH vitamin D2 and 25-OH vitamin D3.

Extraction of low level testosterone and androstenedione from human serum samples using ISOLUTE SLE+
The method in this application has been optimized to extract testosterone and androstenedione from female clinical serum samples. It has achieved very low limits of quantitation for testosterone and androstenedione 0.4 nmol/L and 0.9 nmol/L respectively. The recoveries for both analytes were greater than 90%.

Extraction of a range of opiates and metabolites from human urine using ISOLUTE SLE+ columns prior to GC-MS analysis
An effective and efficient supported liquid extraction protocol for the clean-up and concentration of a range of forensically significant opiates and their metabolites is demonstrated in this application.

Extraction of cocaine and metabolites from urine using ISOLUTE SLE+ prior to LC-MS/MS analysis
Effective and efficient ISOLUTE SLE+ protocols optimized for sample (urine) volumes of either 200 uL or 1 mL are described in this application note. Procedures for high throughput (96-well plate) and column format are included.